The two top diplomats addressed "reports of concerning Russian military activity in and near Ukraine and their continued ironclad commitment to Ukraine's sovereignty and territorial integrity," the department's spokesman Ned Price said in a statement.
The US and the European Union have voiced increasing alarm over recent Russian troop movements near the country's border with Ukraine.
Russian troops edging closer to Ukraine border
Ukrainian President Volodymyr Zelenskiy has said there are almost 100,000 Russian soldiers in the border region.
After discussing the issue of Russian military buildup near Ukraine with US President Joe Biden earlier this week, European Commission President Ursula von der Leyen said EU officials "fully support the territorial integrity of Ukraine."
The Russian troop movements have spurred fears of a possible attack.