The announcement effectively prohibits the three major vaccines that have so far been given the green light around the world.
The German BioNTech vaccine is co-produced by the American firm Pfizer, the Moderna vaccine is a US achievement and the Oxford University-AstraZeneca vaccine — cheaper than the other two options — is from the UK.
Khamenei though praised on-going efforts to produce a vaccine inside Iran.
Iran has been particularly hard-hit by the coronavirus pandemic with almost 56,000 deaths since the virus reached the country.
No rush to negotiate with the US
Khamenei also expressed a lack of interest in the US returning to the nuclear deal which was abandoned by Trump, although he insisted that sanctions be lifted.
"We are in no rush and we are not insisting on [the US's] return. Our demand... is the lifting of sanctions," Khamenei said.
President Rouhani has previously expressed openness to working with the new US president.
ab/rt (Reuters, AFP)
Correction: A previous version of this article stated that Iran was working with the Chinese company Fosun Pharma to produce and distribute vaccines. This could not be verified and has now been removed. The department apologizes for the error.